en Genetics Genetics پژوهشي Original Article <h3 style="margin-top: 3px; text-align: justify;"><span style="line-height:2;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong>Background: </strong>This study evaluated long non-coding RNA ANRIL expression in peripheral blood leukocytes of patients with breast cancer and its diagnostic value in relation to oxidative stress and inflammation.<br> <strong>Methods: </strong>This case&ndash;control study included 29 newly diagnosed patients with breast cancer and 30 healthy controls. ANRIL expression was quantified by quantitative real-time polymerase chain reaction. Malondialdehyde, total antioxidant capacity, superoxide dismutase, and plasma interleukin-6 were measured using commercial assays. Diagnostic performance was assessed by receiver operating characteristic analysis. The Cancer Genome Atlas Breast Invasive Carcinoma data were analyzed for differential expression, co-expression, oxidative stress-related gene correlations, and functional enrichment.<br> <strong>Results:</strong> ANRIL expression showed a decreasing trend in patients with breast cancer versus controls (P = 0.05). Patients had higher malondialdehyde and interleukin-6 levels and lower total antioxidant capacity and superoxide dismutase activity (all P < 0.001). No significant correlations were observed between ANRIL and oxidative stress markers or plasma interleukin-6. Receiver operating characteristic analysis showed good discrimination by ANRIL (area under the curve = 0.864). In The Cancer Genome Atlas Breast Invasive Carcinoma data, ANRIL and interleukin-6 showed a weak but significant inverse correlation (r = &minus;0.114, P < 0.001). Functional enrichment indicated that ANRIL-associated genes were mainly involved in cell-cycle regulation and mitosis.<br> <strong>Conclusions: </strong>ANRIL showed a decreasing trend and good discriminatory performance in breast cancer. Although no direct association was observed with the assessed oxidative stress or inflammatory markers, transcriptomic analysis linked ANRIL to cell-cycle- and mitosis-related pathways. Larger multicenter and mechanistic studies are needed to validate these findings.</span></span></span></h3> Breast cancer , ANRIL , Long non-coding RNA , Biomarker , Oxidative stress , Inflammation, IL-6, qRT-PCR, TCGA-BRCA, Cell cycle regulation 96 106 http://ijbc.ir/browse.php?a_code=A-10-2458-1&slc_lang=en&sid=1 Dhifaf Saleem Kareem Maliki defafsaleem@gmail.com 100319475328460013507 100319475328460013507 No Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Tayebeh Azramezani Kopi @yahoo.com 100319475328460013508 100319475328460013508 No Department of Clinical Biochemistry, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Masoumeh Karimi Karimirt@.com 100319475328460013509 100319475328460013509 No Department of Radiation Oncology, Faculty of Paramedical Scienses, Babol University of Medical Sciences, Babol, Iran. Masoumeh Rezaei Talarposhti 100319475328460013510 100319475328460013510 No Molecular and Cell biology Research Center,Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Abbas Khonakdar-Tarsi khonakdarab@gmail.com 100319475328460013511 100319475328460013511 Yes Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran & Molecular and Cell biology Research Center,Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Hadis Musavi msc.musavi66@gmail.com 100319475328460013512 100319475328460013512 No Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran & Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran.