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:: Volume 1, Issue 4 (Summer 2009) ::
IJBC 2009, 1(4): 151-157 Back to browse issues page
Studies of Methemoglobin Concentrations of Three Human Erythrocyte Genotypes (Hb AA, Hb AS, and Hb SS) in the Presence of Five Antimalarial Drugs
Paul C Chikezie * , Comfort C Monago , Augustine Uwakwe
Abstract:   (9084 Views)

Background: Malaria remains the world’s most devastating human parasitic infection. Our goal was to assess the capacity of increasing concentrations of five antimalarial drugs (FansidarTM, HalfanTM, Quinine, CoartemTM and Chloroquine phosphate) to elicit the generation of methemoglobin in three human erythrocyte genotypes (Hb AA, Hb AS and Hb SS).

Materials and Methods: Spectrophotometric method was used for determination of plasma methaemoglobin concentration in the presence of 0.2 g%, 0.4 g%, 0.6 g% and 0.8 g% (w/v) of the five antimalarial drugs.

Results: The five antimalarial drugs showed a concentration dependent variability to cause the elevation of plasma methemoglobin concentration in the three genotypes. Specifically, CoartemTM, exhibited the highest propensity to elevate plasma methemoglobin concentration. However, the other four antimalarial drugs showed a statistically significant (P<0.05) but minimal effect to cause elevation of plasma methemoglobin concentration. For instance, with Hb AS blood sample and at drug concentration of 0.8g%, methaemoglobin concentrations (percentage) 0f 3.03±1.82, 2.65±0.45, 6.41±1.21, and 3.02±0.98 were obtained for halfan, quinine, coartem and chloroquine phosphate, respectively. The control value was 2.17±1.82% of methemoglobin.

Conclusion: The oxidative potentials of these four antimalarial drugs and their metabolites in the red cells did not overwhelm the erythrocyte methemoglobin reducing capacity that could elicit the presentation in vitro toxic methemoglobinemia.

Keywords: Chloroquine, Coartem, Erythrocyte, Fansidar, Genotype, Halfan, Quinine, Methemoglobin
Full-Text [PDF 186 kb]   (4310 Downloads)    
: Research | Subject: Pediatric Hematology & Oncology
Received: 2011/01/26
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Chikezie P C, Monago C C, Uwakwe A. Studies of Methemoglobin Concentrations of Three Human Erythrocyte Genotypes (Hb AA, Hb AS, and Hb SS) in the Presence of Five Antimalarial Drugs. IJBC. 2009; 1 (4) :151-157
URL: http://ijbc.ir/article-1-5-en.html


Volume 1, Issue 4 (Summer 2009) Back to browse issues page
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
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