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جلد 9 شماره 1 صفحات 12-17 برگشت به فهرست نسخه ها
Expression Pattern of Interferon-γ in Human Leukemic T Cell Lines Following Treatment with Phytoheamagglutinin, phorbol myristate acetate and Lipopolysaccharide
چکیده:   (258 مشاهده)

Background: As a T helper type 1 (Th1) derived cytokine, Interferon gamma (IFN-γ) is an important regulator of inflammatory immune responses. Furthermore, IFN-γ plays an essential role in defense against tumors and intracellular pathogens. This study was designed to assess the pattern of IFN-γ production in human leukemic (Jurkat and Molt-4) T cell lines in vitro.
Methods: Jurkat and Molt-4 cells were cultured in whole RPMI-1640 media. The cells were imbedded at a density of 2×106 cell/ml. The cells were stimulated with different concentrations of Phytoheamagglutinin (PHA) (2-10 µg/ml), phorbol myristate acetate (PMA) (1-25 ng/ml) or lipopolysaccharide (LPS) (1-4 μg/ml) for activation and cytokine production for 48 hours. Then the cell-conditioned media were used for IFN-γ assay. Analysis of variance (ANOVA) was done for comparing the groups statistically.
Results: PHA and PMA substantially augmented IFN-γ level in human leukemic T cells (Molt-4 and Jurkat) in a dose-dependent manner after 48 hours of incubation compared with untreated control cells, whereas LPS did not have any significant effect on IFN-γ production in human leukemic T cell lines compared with unstimulated cells.
Conclusion: human leukemic Jurkat and Molt-4 T cell lines could potentially produce IFN-γ with different amounts. PHA was a more potent stimulator of IFN-γ production than PMA. Molt-4 cell line could produce more IFN-γ than Jurkat cell line. These cells could be appropriate for studying the mechanisms of action of immunomodulators as well as screening the IFN-γ stimulators/inhibitors.

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: پژوهشي | موضوع مقاله: Immunology
دریافت: ۱۳۹۵/۷/۲۸ | پذیرش: ۱۳۹۵/۱۱/۵ | انتشار: ۱۳۹۶/۱/۱۴
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Hajighasemi F, Mirshafiey A. Expression Pattern of Interferon-γ in Human Leukemic T Cell Lines Following Treatment with Phytoheamagglutinin, phorbol myristate acetate and Lipopolysaccharide. IJBC. 2017; 9 (1) :12-17
URL: http://ijbc.ir/article-1-693-fa.html
Expression Pattern of Interferon-γ in Human Leukemic T Cell Lines Following Treatment with Phytoheamagglutinin, phorbol myristate acetate and Lipopolysaccharide. مجله خون و سرطان ايران. 1395; 9 (1) :12-17

URL: http://ijbc.ir/article-1-693-fa.html

دوره 9، شماره 1 - ( 12-1395 ) برگشت به فهرست نسخه ها
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
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