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Volume 12, Issue 4 ( December 2020 2020)
Iranian Journal of Blood and Cancer 2020, 12(4): 131-137 |
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Pudineh Moarref M, Safavi M, Mostafavi M, Karami-Mohajeri S. Sub-acute Exposure to Benzene Accelerates the Aging Process of Red Blood Cells; an In vivo Study. Iranian Journal of Blood and Cancer 2020; 12 (4) :131-137
URL: http://ijbc.ir/article-1-996-en.html
URL: http://ijbc.ir/article-1-996-en.html
Mokarameh Pudineh Moarref1 
, Moeinadin Safavi2 , Mohammad-Amin Mostafavi1 , Somayyeh Karami-Mohajeri * 3
, Moeinadin Safavi2 , Mohammad-Amin Mostafavi1 , Somayyeh Karami-Mohajeri * 3
1- Department of Toxicology and Pharmacology, Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
2- Department of Pathology, Afzalipour Medical Faculty, Kerman University of Medical Sciences, Kerman, Iran
3- Department of Toxicology and Pharmacology, Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran , Somayyehkarami@gmail.com
2- Department of Pathology, Afzalipour Medical Faculty, Kerman University of Medical Sciences, Kerman, Iran
3- Department of Toxicology and Pharmacology, Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran , Somayyehkarami@gmail.com
Abstract: (2957 Views)
Background: The well-known toxic effects of benzene toxicity are bone marrow depression, reduction in blood cell counts, and induction of leukemia and aplastic anemia. This study was designed to evaluate biomarkers of aging in red blood cells (RBCs).
Methods: Mice were exposed to benzene (50, 100, and 200 mg/kg/day) orally for 28 days. A group of benzene-exposed mice were injected intraperitoneally with N-acetylcysteine (NAC, 150 mg/kg/day). Hematological factors, erythrocyte morphology, and sialic acid content of RBCs along with oxidative stress biomarkers were investigated.
Results: Benzene dose-dependently reduced RBCs count, hemoglobin level, RBCs membrane sialic acid levels, the total antioxidant capacity of plasma, and G6PD activity of RBCs. The activity of antioxidant enzymes and lactate dehydrogenase, oxidative damage end-products and bilirubin levels, reticulocyte count, and RDW and MCV ranges increased in a dose-dependent manner. Poikilocytosis (spherocyte, burr cell, schistocyte and blister cell) and anisocytosis were observed in high doses of benzene.
Conclusion: Our results support the acceleration of RBCs aging and hemolytic anemia in mice exposed to benzene. Co-administration of NAC as an antioxidant effectively alleviated hematotoxicity of benzene.
Methods: Mice were exposed to benzene (50, 100, and 200 mg/kg/day) orally for 28 days. A group of benzene-exposed mice were injected intraperitoneally with N-acetylcysteine (NAC, 150 mg/kg/day). Hematological factors, erythrocyte morphology, and sialic acid content of RBCs along with oxidative stress biomarkers were investigated.
Results: Benzene dose-dependently reduced RBCs count, hemoglobin level, RBCs membrane sialic acid levels, the total antioxidant capacity of plasma, and G6PD activity of RBCs. The activity of antioxidant enzymes and lactate dehydrogenase, oxidative damage end-products and bilirubin levels, reticulocyte count, and RDW and MCV ranges increased in a dose-dependent manner. Poikilocytosis (spherocyte, burr cell, schistocyte and blister cell) and anisocytosis were observed in high doses of benzene.
Conclusion: Our results support the acceleration of RBCs aging and hemolytic anemia in mice exposed to benzene. Co-administration of NAC as an antioxidant effectively alleviated hematotoxicity of benzene.
: Original Article |
Subject:
Pathology
Received: 2020/04/24 | Accepted: 2020/11/2 | Published: 2020/12/31
Received: 2020/04/24 | Accepted: 2020/11/2 | Published: 2020/12/31
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