TY - JOUR T1 - Evaluation of Leukoreduction in Packed Cell Units Filtered by Home-Made Bedside Filters: Pre and Post Revision of Product Technology and Materials According to Standard Values TT - JF - IJBC JO - IJBC VL - 1 IS - 1 UR - http://ijbc.ir/article-1-29-en.html Y1 - 2008 SP - 17 EP - 21 KW - leukoreduction KW - Filtration KW - flowcytometry KW - true count beads KW - anti CD45 antibody N2 - Introduction: Leukocytes causing a wide variety of side effects after transfusion are present in all blood products prepared by standard methods. As a consequence, the use of filter technology for leukoreduction has been widely practiced .According to AABB accreditation in 1996, leukoreduced blood components must contain less than 5×106 leukocytes per unit, but sometimes this value is higher even in leukoreduced products. In this study we did absolute leukocyte count in filtered (home made bedside filter) packed cell units by two methods of true count as standard method and CD45 MoAb. Materials & Methods: 93 packed cell units were stored at 4°c and filtered by two types of home-made filters according to manufacturer's instructions. Furthermore, eight packed cell units were filtered by Europe certified control group filters (bio-fil). Sample preparation was done according to True count kit and CD45 MoAb procedures and analysis was performed by flowcytometry (EPICS-XL,coulter) and (Partec PAS III).The results were then analyzed by chi2 test via SPSS. Results: The mean values of leukocyte count/unit by anti CD45 and True count method were 9×106 and 10×106 respectively in 55 pre-revised filter bags these figures were 4.2×106 and 4.8×106 in 30 post-revised filters, whereas the mean leukocyte count/bag in eight control filters was 2.3×106,we selected randomly eight test units out of 53 and 30 pre-revised and post-revised filtered bags, respectively(equal with number of units in the control group) to compare the test and control groups The mean values of leukocyte count/bag in pre-revised test group was 7.9±5.4×106 and in postrevised test group was 4.2 ×106 but in the control group it was 2.3 ×106 (p value M3 ER -