%0 Journal Article %A Mohammadi, S %A Shaiegan, M %A Moghadam, KA %A Babaee, GR %A Samiei, S %A Ghavamzadeh, A %A Shakerpour, F %T Minor Histocompatibility Antigen HA-1 Frequency and Acute GVHD in Some Hematopoietic Stem Sell Transplanted Iranian Patients %J Iranian Journal of Blood and Cancer %V 1 %N 1 %U http://ijbc.ir/article-1-32-en.html %R %D 2008 %K HA-1 disparity, GVHD, Hematopoietic Stem cell transplantation (HSCT), Iranian, %X Background: It is suggested that HA-1 mismatching among hematopoietic stem cell transplanted recipients-donors is associated with acute graft-versus-host disease (aGVHD). So the aim of this study was to evaluate HA-1 frequency and examine the correlation between HA-1 disparity and GVHD patients who received transplantation from a HLA-identical sibling. Samples and Methods: the DNA was extracted from 30 pairs of HLA-A2-positive Iranian recipients-donors with GVHD I-IV and 25 pairs without GVHD. All the patients received HSCT from HLA-identical siblings. HA-1 was detected by SSP-PCR method. The HA-1 typing was performed using SSP method (SSP Minor Histocompatibility Antigen primer sets). Results: The frequency of HA-1R and HA-1H alleles in patients were 0.55 and 0.45 respectively, and showed no significant difference with these alleles frequency in donors 0.53 and 0.47 respectively (p>0.05). The HA-1 disparity was detected in 8 out of 55 donor/recipient pairs (14.5%). The aGVHD (grades I-IV) occurred in 6 (75%) out of 8 patients but in spite of higher incidence of it in the group of patients with HA-1 incompatibility, it was not statistically significant. Conclusion: In spite of higher frequency of HA-1 disparity in GVHD+ group our data did not reflect a significant association between the HA-1 disparity and risk of acute GVHD. %> http://ijbc.ir/article-1-32-en.pdf %P 33-38 %& 33 %! %9 Original Article %L A-10-2-32 %+ %G eng %@ 2008-4595 %[ 2008