Iranian Journal of Blood and Cancer
Iranian Journal of Blood and Cancer
Medical Sciences
http://ijbc.ir
1
admin
2008-4595
2008-4609
8
10.61186/ijbc
14
2008-4595
13
en
jalali
1395
3
1
gregorian
2016
6
1
8
2
online
1
fulltext
en
Expression Analysis of Foxo3a Gene in Pediatric Acute Lymphoblastic Leukemia in Southern Iranian Population
Pediatric Hematology & Oncology
Pediatric Hematology & Oncology
پژوهشي
Original Article
<p>Background: Acute lymphoblastic leukemia (ALL), the most common childhood cancer with a peak incidence in children from 2-5 years old, might be associated with poor prognosis and resistance to therapy in specific cytogenetic backgrounds. FoxO3a, a member of the forkhead class ‘O’ (FoxO) transcription factors, is a main downstream target of PI3K/AKT pathway which regulates different variety of biological processes and is overactivated in several human cancers. We aimed to evaluate the aberration of the FoxO3a gene in mRNA level in childhood ALL and compare them with healthy controls. <br>
Methods: Real-time quantitative RT-PCR (qRT-PCR) was used to detect FOXO3a expression in 30 new cases of pediatric ALL and 30 age- and sex-matched healthy children as the control group. <br>
Results: the expression level of the FoxO3a gene was significantly lower in ALL patients compared to healthy controls (P<0.0001), while no difference was observed between the two sub-types B- and T-ALL. <br>
Conclusion: Our study suggested that decreased FoxO3a expression may play an important role in the development of pediatric ALL. FoxO3a could be considered as a molecular target of therapy in ALL malignancy.</p>
FoxO3a, Pediatric ALL, Gene expression, PI3K/AKT pathway
47
51
http://ijbc.ir/browse.php?a_code=A-10-134-1&slc_lang=en&sid=1
Malihe
Mirzaei
ملیحه
میرزایی
10031947532846003347
10031947532846003347
No
Department of Biology, Islamic Azad University, Arsanjan branch, Arsanjan, Iran
دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد ارسنجان
Mahboobeh
Nasiri
محبوبه
نصیری
10031947532846003348
10031947532846003348
Yes
Department of Biology, Islamic Azad University, Arsanjan branch, Arsanjan, Iran
دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد ارسنجان
Mehran
Karimi
مهران
کریمی
10031947532846003349
10031947532846003349
No
Department of Biology, Islamic Azad University, Arsanjan branch, Arsanjan, Iran
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran