en Adults Hematology & Oncology Adults Hematology & Oncology پژوهشي Original Article <p style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Arial;"><span style="font-size:14px;"><b><span lang="EN-GB">Background</span></b><span lang="EN-GB">: Aberrant expression of cross-lineage antigens gives valuable insight into the diagnosis and prognosis of acute leukemia. In countries like India, cytogenetic tests are widely accessible. Exploring the prognostic value of an accessible test is of great importance. Therefore, establishing a population-specific immunophenotype database will enable to design an antibody panel equipped to detect cross-lineage antigen expression. The aim of this study was to determine the frequencies of cross-lineage antigen expression in Acute Lymphoblastic Leukaemia (ALL), its relationship with clinical features, and changes in blood counts during the treatment course. </span><br> <b><span lang="EN-GB">Methods</span></b><span lang="EN-GB">: This was a retrospective observational study conducted at a tertiary care hospital. Consecutive ALL cases over 2 years were reviewed. Relation of cross-lineage aberrant antigens with blood counts and clinical features were studied. Chi-square test and Fisher&rsquo;s exact test were used. </span><br> <b><span lang="EN-GB">Results</span></b><span lang="EN-GB">: A total of 149 ALL cases were included in the study. Thirty (20.1%) cases showed expression of cross-lineage antigens. CD7 was the most commonly expressed cross-lineage antigen, seen in 14 (10.5%) cases of B-ALL. CD13, seen in eight (5.3%) patients, was the most frequent aberrant myeloid antigen. Myeloid aberrancies were associated with lower WBC count and blast count while aberrancies of T-cell antigens on B-ALL showed higher WBC count and blast count.</span><br> <b><span lang="EN-GB">Conclusions</span></b><span lang="EN-GB">: Cross lineage antigenic aberrancies influence blast count and WBC count. Documentation of these aberrancies in ALL helps in prognostication and monitoring of the disease.</span></span></span></span></p> Acute lymphoblastic leukemia, cross lineage aberrant antigen expression, multiparametric flow cytometry, immunophenotyping 22 31 http://ijbc.ir/browse.php?a_code=A-10-844-1&slc_lang=en&sid=1 Sindhura Lakshmi Koulmane Laxminarayana sindhura.lakshmi@manipal.edu 10031947532846008861 10031947532846008861 No Associate Professor, Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal - 576104, KA, India. Aradhana Harrison aradhanaharrison@gmail.com 10031947532846008862 10031947532846008862 Yes Assistant Professor, Department of Pathology, Melaka Manipal Medical College, Manipal, Manipal Academy of Higher Education, Manipal - 576104, KA, India. Phaneendra VR Datari drphaneendradatari@outlook.com 10031947532846008863 10031947532846008863 No Former Post graduate student, Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal - 576104, KA, India. Sushma V Belurkar sushma.b@manipal.edu 10031947532846008864 10031947532846008864 No Additional Professor, Department of Pathology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal - 576104, KA, India.