en Immunology Immunology پژوهشي Original Article <p style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Arial;"><span style="font-size:14px;"><b><span style="color:#0d0d0d">Introduction: </span></b><span style="color:#0d0d0d">CLL is one of the most common leukemias, which is categorized by the accumulation of mature CD5⁺ B-lymphocytes in the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, the status of rs6449182 polymorphism of the CD38 gene and its association with clinical and laboratory parameters of CLL patients was evaluated.</span><br> <span style="color:#0d0d0d">&nbsp;<b>Methods:</b> Genomic DNA extraction was performed using the salting out method. The CD38 gene polymorphism (rs6449182) was studied in 70 patients with CLL and 70 healthy individuals using the PCR-RFLP method. </span><br> <b><span style="color:#0d0d0d">Results:</span></b><span style="color:#0d0d0d"> The results of this study showed that the control group had 86% wild-type rs6449182 (CC), 12% heterozygous (CG), and 2% homozygous (GG) genotypes. In the case group, 62% had wild-type genotype (CC) 26% were heterozygous (CG), and 12% were homozygous (GG). Statistical analysis showed that the heterozygous genotype for the CD38 gene&nbsp; was significantly associated with CLL. It was also understood that this polymorphism had a significant relationship with hemoglobin, age, and organomegaly of patients. </span><br> <b><span style="color:#0d0d0d">Conclusions:</span></b><span style="color:#0d0d0d"> The CD38 gene polymorphism of rs6449182 SNP G allele had the highest frequency. Moreover, based on the results, this polymorphism has a significant relationship with organomegaly, which indicates the importance of these markers in the pathogenesis and prognosis of the disease.</span><br> <b><span style="color:#0d0d0d">Keywords:</span></b><span style="color:#0d0d0d"> Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38</span><br> &nbsp;</span></span></span></p> Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38 16 21 http://ijbc.ir/browse.php?a_code=A-10-855-1&slc_lang=en&sid=1 Morteza Hashemi Morteza.H@yahoo.com 10031947532846008857 10031947532846008857 No Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran. Hadi Esmaeili gouvarchin ghaleh h.smaili69@yahoo.com 10031947532846008858 10031947532846008858 No Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran. Shahrbano Rostami Shahrbano.R@yahoo.com 10031947532846008859 10031947532846008859 No Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran. Bahman Jalali kondori bahmanjalali2010@gmail.com 10031947532846008860 10031947532846008860 Yes Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.