en Adults Hematology & Oncology Adults Hematology & Oncology پژوهشي Original Article <span style="line-height:2;"><span style="font-size:14px;"><span style="font-family:Arial;"><span style="color:black"><b><span lang="EN-GB">Objective:</span></b><span lang="EN-GB"> Many studies have suggested </span>Mesenchymal stem cells, as a promising way to develop new treatment strategies for different types of disease. However, due to their possible tumorigenic effects, their clinical use has been limited. Since a great deal of MSCs&rsquo; therapeutic benefits depend on MSC-derived extracellular vesicles, these particles have been receiving much attention in the past couple of years.<span lang="EN-GB"> With this in mind</span>,<span lang="EN-GB"> we aimed to study the Effects of both peripheral blood mononuclear cells and human umbilical cord MSC-derived extracellular vesicles on cell growth, proliferation, and apoptosis of the Nalm6 cell line.</span></span><br> <span style="color:black"><b>Materials and Methods: </b>Isolated HUCMSCs were cultured, and PBMCs were acquired using the Ficoll-Hypaque technique. Their EVs were then extracted<span lang="EN-GB">.</span> The Nalm6 cells were divided into five groups: a control group and four treatment groups, which were treated with MSC- and MNC-derived EVs at different concentrations. <span lang="EN-GB">Cell viability and metabolic assays were evaluated using trypan blue staining and the MTT assay, respectively. Thereafter, a flow cytometric assay was performed to detect cell cycle progression and apoptosis.</span></span><br> <span style="color:black"><b>Results:</b><span lang="EN-GB"> Our research revealed that the level of metabolic activity between Nalm6-treated EVs and the control group was not significantly different after 3 days. Also, no significant changes were reported in the growth and apoptosis effects of EVs on Nalm6 cells compared to the control group. Furthermore, different concentrations of EVs didn&rsquo;t cause any inhibition on tumor growth.</span></span><br> <span style="color:#000000;"><strong>Conclusion:</strong>Obtained EVs neither showed any anti-tumor effect nor caused any progression or aggressiveness in the Nalm6 cell line.</span></span></span></span> Mesenchymal Stem Cells, Extracellular Vesicles, Umbilical Cord, Cell Survival, Cell Cycle. 78 88 http://ijbc.ir/browse.php?a_code=A-10-949-1&slc_lang=en&sid=1 Hajar Mardani Valandani hajarmardani61@gmail.com 100319475328460010455 100319475328460010455 No Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences Kerman University of Medical Sciences, Kerman, Iran Amir mohammad zahedi amirzahedi917@gmail.com 100319475328460010456 100319475328460010456 No Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences Kerman University of Medical Sciences, Kerman, Iran Roohollah Mirzaee Khalilabadi Khalilabadi60@gmail.com 100319475328460010457 100319475328460010457 Yes Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medical Sciences Kerman University of Medical Sciences, Kerman, Iran