en Adults Hematology & Oncology Adults Hematology & Oncology پژوهشي Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:2;"><span style="color:black"><b>Background:</b> Colorectal cancer (CRC), a common and aggressive gastrointestinal cancer, presents significant challenges in diagnosis and prognosis prediction despite available detection and treatment options. Many studies emphasized the crucial link between abnormal microRNA regulation and their potential role in cancer development and progression. These miRNAs are recognized as important non-invasive biomarkers for prognosis and overall survival prediction in various cancers, including CRC. </span><br> <span style="color:black"><b>Materials and Methods:</b> In this study, we compared the expression patterns of eight miRNAs in the serum of 36 CRC patients with those of 37 healthy controls. The matching criteria included clinicodemographic factors and CRC susceptibility, and the analysis was performed using quantitative real-time PCR (qRT-PCR). </span><br> <span style="color:black"><b>Results: </b>The serum miRNA levels of these eight miRNAs (miR-19a, miR-92a, miR-103, miR-106a, miR-107a, miR-150, miR-221, and miR-720) in the study groups are significantly higher compared to the control group. This analysis revealed eight specific miRNAs with varying expression levels in CRC patients.&nbsp; Furthermore, bioinformatic analysis using data collection and analytical tools has shown that these miRNAs may be associated with important aspects of colorectal cancer development and progression through the PI3K/AKT/PTEN, WNT/CATENIN, and EMT signaling pathways.</span><b>Conclusion:</b> Our analysis has identified a group of 8 overexpressed miRNAs (miR-19a, miR-92a, miR-103, miR-106a, miR-107a, miR-150, miR-221, and miR-720.) in serum samples of CRC patients. <b>:</b> Although further validation in larger and more diverse groups is necessary,&nbsp; these findings support a potential mechanism of action for these miRNAs in CRC and their association with essential signaling pathways, including PI3K/AKT/PTEN, WNT/CATENIN, and EMT.</span></span></span></div> Colorectal cancer (CRC), microRNAs (miRNAs), Biomarkers, Prognosis, PI3K/AKT/PTEN, WNT/CATENIN, EMT 7 14 http://ijbc.ir/browse.php?a_code=A-10-1144-1&slc_lang=en&sid=1 Meral Merve Oğuz monica.khademi@yahoo.com 100319475328460010885 100319475328460010885 No Department of Internal Medicine, Pamukkale University, Denizli, Turkey/ The Affiliated Hospital of Pamukkale University. Ehteram Khademi Siahestalkhi e.khademi18@gmail.com 100319475328460010886 100319475328460010886 Yes Department of Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey. Arzu Yaren ayaren@pau.tr 100319475328460010887 100319475328460010887 No Department of Internal Medicine, Pamukkale University, Denizli, Turkey/ The Affiliated Hospital of Pamukkale University. Aydin Demiray ademiray@pau.edu.tr 100319475328460010888 100319475328460010888 No Department of Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey. Atike Gökçen Demiray agakaslan@pau.edu.tr 100319475328460010889 100319475328460010889 No Department of Internal Medicine, Pamukkale University, Denizli, Turkey/ The Affiliated Hospital of Pamukkale University.