Complete Content of this Issue and Cover Content 2 0 0 2015/12/16 1394/9/25 2015/12/16 1394/9/25 ## ##

Bone Involvement in Neuroblastoma 2 203 205 2015/12/162015/12/13 1394/9/22 2015/12/162015/12/13 1394/9/22 Fatima Malek Fatima Malek fmalek7721@gmail.com Samin Alavi Samin Alavi ## ##

Treatment of Newly Diagnosed and Relapsed Hodgkin Lymphoma in Children 2 2 Hodgkin lymphoma (HL) accounts for about 10% of all childhood cancers. Five-year survival rates with modern therapies are now approaching >90-95% as a consequence of its significant sensitivity to both chemotherapy and radiation. The current challenge is to determine how much therapy is needed to improve survival and how to adapt treatment to the patient to prevent these long term toxicities. This challenge has resulted in the development of different strategies aimed at recognizing the optimal balance between preserving overall survival and avoidance of long-term treatment-related morbidity. Strategies in children could be quite different from those used for adults with HL. More defined risk stratification through imaging studies and biologic markers have been developped. Increased knowledge of the biology of HL have led to the introduction of targeted therapies in both the frontline and relapsed patients. Collaborative multicenter studies are required to develop new combination therapies for children with newly diagnosed and refractory HL. 206 212 2015/12/162015/12/132015/12/13 1394/9/22 2015/12/162015/12/132015/12/13 1394/9/22 Odile Oberlin Odile Oberlin Odile.oberlin@gustaveroussy.fr Catherine Patte Catherine Patte Diagnosed Treatment Children ## ##

Methylation Status of SOX17 and RUNX3 Genes in Acute Leukemia تغییر در مسیر سیگنالی Wnt از طریق متیلاسیون ژن های SOX17 و RUNX3 در بیماران لوسمی حاد 2 2 Background: Several studies have examined the presence of DNA methylation of CpG islands in leukemia. Methylation of SOX17 and RUNX3 genes may play a role in leukemogenesis through silencing tumor suppressor genes. We investigated the methylation status of SOX17 and RUNX3 genes in patients with acute leukemia. Methods: In this case-control study, peripheral blood samples from 100 AML and 100 ALL patients and 100 healthy controls were collected. Isolated DNA was treated with sodium bisulfite and methylation status was examined by methylation specific PCR (MS-PCR) with primers specific for methylated and unmethylated sequences of SOX17 and RUNX3 genes. Results: The frequency of hypermethylation of SOX17 and RUNX3 genes were 36% and 28%I in patients with acute myeloid leukemia (AML), and 21% and 22% in patients with acute lymphoblastic leukemia (ALL), respectively. Aberrant methylation of these genes was found in all FAB classifications of AML and ALL. Hypermethylation of SOX17 (P=0.055) and RUNX3 (P=0.003) genes were associated with FAB-M0 and M1 subtypes of AML, respectively. Also, aberrant methylation of RUNX3 gene was associated with FAB-L1 subtype of ALL (P=0.053). There was not any significant association between hypermethylation of SOX17 and RUNX3 genes and clinical parameters of patients with leukemia including sex, age, WBC, and platelet counts. Conclusion: Hypermethylation of SOX17 and RUNX3 genes was seen in patients with acute leukemia. Moreover, no significant association was observed between hypermethylation of SOX17 and RUNX3 and induction of remission. 213 219 2015/12/162015/12/132015/12/132015/10/27 1394/8/5 2015/12/162015/12/132015/12/132015/11/27 1394/9/6 علی قاسمی Ali Ghasemi 1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran a.ghotaslou@gmail.com عباس قوطاسلو Abbas Ghotaslou 2. Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran a.ghotaslou@gmail.com کاظم غفاری Kazem Ghaffari 3. Department of Hematology, School of Allied Medical Sciences, Arak University of Medical Sciences, Arak, Iran a.ghotaslou@gmail.com محسن محمدی Mohsen Mohammadi 4. Department of Biotechnology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran a.ghotaslou@gmail.com Acute myeloid leukemia Acute Lymphoblastic leukemia RUNX3 SOX17 DNA Methylation لوسمی حاد میلوئیدی لوسمی حاد لنفوبلاستیک RUNX3 SOX17 DNA Methylation ## ##

Correlation Between Demographic and Laboratory Variables in Adult Patients with Acute Idiopathic Thrombocytopenic Purpura in West Iran بررسی متغیرها در بیماران Acute ITP 2 2 Background: Idiopathic thrombocytopenic purpura is an autoimmune blood disorder in which platelet destruction is mediated by anti-platelet antibodies. There are two forms of ITP: acute and chronic. The aim of the present study was to evaluate the clinical variables in adult patients with acute ITP in west of Iran. Patients and Methods: Medical records of adult patients with diagnosis of acute ITP referring to Hematology Clinic of Kermanshah from year 2004-2014 were analyzed. Demographic and hematologic data and status of H pylori infection of the patients were extracted. Results: There were records of fifty-three patients diagnosed with acute ITP. Mean age at diagnosis was 39.1 years (±13.3) ranging from 14-68 years. Twenty patients (37.7%) were male. Out of 53 patients, 25 cases (47.2%) were positive for H pylori infection. There was significant association between Hb and platelet with sex of the patients (P;le0.05). Conclusions: Mean age of adult patients with acute ITP was more than figures expected in chronic ITP patients. In addition, Prevalence of H pylori infection in acute ITP patients was more than chronic ITP patients. 220 222 2015/12/162015/12/132015/12/132015/10/272015/04/29 1394/2/9 2015/12/162015/12/132015/12/132015/11/272015/06/28 1394/4/7 Mehrdad Payandeh Mehrdad Payandeh Department of Hematology and Medical Oncology, Kermanshah University of Medical Sciences, Kermanshah, Iran md.payandeh@yahoo.com Masoud Sadeghi Masoud Sadeghi Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran sadeghi_mbrc@yahoo.com Edris Sadeghi Edris Sadeghi Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran sadeghi_mkn@yahoo.com Mostafa Hossaini Mostafa Hossaini Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran mostafahossaini@yahoo.com Idiopathic thrombocytopenic purpura Helicobacter pylori infection Acute Chronic Hemoglobin WBC count ## ##

The Prognostic Value of White Blood Cells Count in Patients with Myocardial Infarction تاثیر مقدار گلبول سفید در بیماران سکته قلبی 2 2 Background: Ischemic heart disease and acute myocardial infarction is one of the most dramatic manifestations in one of the most investigated fields in the past few decades. In this study, the prognostic value of white blood cells count in patients with myocardial infarction (MI) was investigated in a six months follow-up. Methods: In this cohort study, 106 patients with MI were investigated. White blood cell counts were assessed 48 hours after MI and the location of MI was determined using ECG. Mortality rate was determined and their correlation with leukocytosis was analyzed up to 6 months of follow-up. Binary logistic regression analysis was applied between factors such as mortality rate, location of the myocardial infarction, sex, hemoglobin and WBC count. Results: Mean age of the patients was 62.5±13.3 years. 76.4% were men. 26% of patients had leukocytosis. Leukocytosis was significantly correlated with mortality in a six-month follow-up period (P<0.001). Fifteen (14.2%) patients died during the first three months of follow-up, of which 13 (86.7%) had leukocytosis. It was also shown that mean age of the patients and anemia in deceased group were significantly more than the survived group. Conclusion: High WBC count in the first 48-h after MI can be regarded as a poor prognostic factor and it has an independent role in determining prognosis of patients with MI for the next six months. 223 226 2015/12/162015/12/132015/12/132015/10/272015/04/292015/06/16 1394/3/26 2015/12/162015/12/132015/12/132015/11/272015/06/282015/12/6 1394/9/15 Ali ShahriariAhmadi Ali ShahriariAhmadi Department of Hematology-Oncology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran shahriariahmadi@gmail.com Abdollah Amirfarhangi Abdollah Amirfarhangi Department of Hematology-Oncology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran abdullah@yahoo.com Abbas Gheisoori Abbas Gheisoori Department of Hematology-Oncology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran gheisoori85245@yahoo.com Mohsen Arabi Mohsen Arabi Department of Hematology-Oncology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran mohsenarabi@yahoo.com Alireza Mahmoudian Alireza Mahmoudian Department of Hematology-Oncology, Rasoul-e-Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran alireza.m@yahoo.com Mehrdad Payandeh Mehrdad Payandeh Department of Hematology-Oncology, Kermanshah University of Medical Sciences, Kermanshah, Iran md.payandeh@yahoo.com Masoud Sadeghi Masoud Sadeghi Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran sadeghi_mbrc@yahoo.com Leukocytosis Mortality Rates Myocardial Iinfarction WBC Count Prognostic factor ## ##

Evaluating Blood Requests and Transfusion Practice in Major Surgical Procedures Evaluating Blood Requests and Transfusion Practices in Widespread Surgical Procedures of Rasht Hospitals, Iran 2 2 Background: Red blood cell and other blood products play a crucial role in management of different disease processes, but in spite of implementation of crucial steps to improve the process of request for blood and blood consumption, there is still not enough information available regarding the proper practice in many medical centres. We aimed to evaluate the status of blood product requests and transfusion practice in surgical patients in Al-Zahra and 17th Shahrivar hospitals, Rasht, Iran. Materials: This retrospective, descriptive cross-sectional study was performed using medical records of patients undergoing major surgical procedures in Al-Zahra and 17th Shahrivar hospitals, Rasht, Iran, from April to December 2013. The cross-match to transfusion ratio (C/T ratio), transfusion probability (T%) and transfusion index (TI) were analyzed. We used SPSS analytical software to analyze the data. Results: Transfusion index was 0.27, transfusion probability 12.8% and C/T ratio was 7.38 which were higher than standards, indicating that only 54 units out of 399 red blood cells units requested were used. Laparoscopic surgery had the worst indicator in terms of wastage of packed cell products. These findings in 17th Shahrivar hospital were 0.09, 8.82% and 12.5, respectively. In this hospital only 10 units out of 125 requested units were used. Appendectomy showed the worst indicators. Conclusion: Blood transfusion indexes particularly for laparoscopic and appendectomy procedures were high in two hospitals studied. In order to overcome this problems, providing teaching courses for proper transfusion practice in surgery departments to improve their knowledge of haemovigilance and preparation of defined guidelines for red blood cells’ cross matching and transfusion is recommended. 227 230 2015/12/162015/12/132015/12/132015/10/272015/04/292015/06/162015/07/13 1394/4/22 2015/12/162015/12/132015/12/132015/11/272015/06/282015/12/62015/12/14 1394/9/23 Sahar Sheikhansari Sahar Sheikhansari Genaral Practitioner, school of medicine, Guilan University of Medical Sciences, Rasht, Iran Bahram Darbandi Bahram Darbandi PediatricHematologist and Oncologist,:Pediatrics Growth Disorders Research Center, 17th Sharivar Hospital, school of medicine, Guilan University of Medical Sciences, Rasht, Iran Ziba Zahiri Sorouri Ziba Zahiri Sorouri Adel Bagheralimi Adel Bagheralimi PediatricHematologist and Oncologist,:Pediatrics Growth Disorders Research Center, 17th Sharivar Hospital, school of medicine, Guilan University of Medical Sciences, Rasht, Iran Blood transfusion Red blood cell Surgery Cross mach haemovigilance Iran Blood request and utilisation ## ##

Metronomic Maintenance Therapy in Refractory Acute Myeloblastic Leukemia with Monosomy 7 اپاندیموما به عنوان بدخیمی ثانویه بعدازدرمان لنفوم غیرهوجکین 2 2 Patients with acute myeloblastic leukemia (AML) with monosomy 7 are a group of patients with refractory AML who have a very poor prognosis. Therefore, rationally designed new therapies, including metronomic chemotherapy regimen with histidine deacetylase inhibitors (Valporic acid, ATRA) are being investigated as potential treatments for the population of refractory cases of AML. Herein, we report a patient with primary refractory AML who was treated with oral low-dose chemotherapy after standard systemic chemotherapy. 231 234 2015/12/162015/12/132015/12/132015/10/272015/04/292015/06/162015/07/132015/03/14 1393/12/23 2015/12/162015/12/132015/12/132015/11/272015/06/282015/12/62015/12/142015/12/14 1394/9/23 Mahdi Shahriari Mahdi Shahriari بابک عبدالکریمی babak abdolkarimi pediatric hematology oncology fellowship b.abdolkarimi@yahoo.com Fazl Saleh Fazl Saleh Pooria Salajeghe Pooria Salajeghe Metronomic Chemotherapy Histone deacetylase Inhibitor Acute Myeloblastic Leukemia Valporic acid All-trans retinoic acid ## ##

Disseminated Intravascular Coagulation in a Case of Brucellosis Misdiagnosed as Thrombotic Thrombocytopenia Purpura کواگولوپاتی مصرفی در بیمار با بروسلوزیس که ابتدا با ترومبوسیتوپنیک ترومبوتیک پورپورا اشتباه شد 2 235 236 2015/12/162015/12/132015/12/132015/10/272015/04/292015/06/162015/07/132015/03/142015/01/24 1393/11/4 2015/12/162015/12/132015/12/132015/11/272015/06/282015/12/62015/12/142015/12/142015/12/14 1394/9/23 Nasim Valizadeh Nasim Valizadeh Tehran University of Medical Sciences nsedaha0@gmail.com TTP DIC Brucellosis DIC TTP Brucellosis ## ##