[Home ] [Archive]    
:: Home :: About Journal :: Registration :: Article Submission :: Contact us :: Site Map ::
:: Volume 10, Issue 4 (December 2018 2018) ::
IJBC 2018, 10(4): 124-129 Back to browse issues page
Cytotoxicity of Metoprolol on Leukemic Cells in Vitro
Baran Hajatbeigi, Fatemeh Hajighasemi *
Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
Abstract:   (442 Views)
Background: β-Blockers have shown considerable cytotoxic, anti-tumor and anti-angiogenic effects. Metoprolol, a β-Blocker with anti-inflammation, anti-tumor and anti-angiogenic properties has been widely used for treatment of some cardiovascular diseases such as angina, hypertension, heart failure and myocardial infraction. Limited data exist about the cytotoxic effects of metoprolol on human cancer cells. The aim of this study was to investigate the cytotoxic effect of metoprolol on U937 and MOLT-4 cells in vitro. 
Methods: Human leukemic T cell (MOLT-4) and monocyte (U937) were cultured in Roswell Park Memorial Institute (RPMI) 1640 complete medium. Then, the cultured U937 and MOLT-4 cells were treated with different concentration of metoprolol (1, 10, 50, 100, 500 and 1000 μg/ml) for 24, 48 and 72 hours. The cytotoxicity of metoprolol was determined by using MTT (3-[4, 5 dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay. 
Results: Metoprolol significantly decreased the viability of U937 and MOLT-4 cells at 1000μg/ml (3740.14µM) concentration after 48 hours incubation time (P<0.01). In addition, metoprolol significantly reduced the viability of U937 cells at ≥500 μg/ml (≥1870.07µM) concentrations after 72 hours incubation time (P<0.001). Moreover, metoprolol significantly decreased the viability of MOLT-4 cells at ≥100 μg/ml (≥374.01µM) concentrations after 72 hours incubation (P<0.001). 
Conclusion: According to the results of this study, metoprolol showed cytotoxic effect on U937 and MOLT-4 cells dose and time dependently. Therefore, metoprolol might have potential implication in therapy of leukemia as well as other malignancies. 
Keywords: Metoprolol, U937, MOLT-4, Cytotoxic
Full-Text [PDF 1219 kb]   (311 Downloads)    
: Research | Subject: Immunology
Received: 2018/10/10 | Accepted: 2018/12/25 | Published: 2019/01/5
* Corresponding Author Address: Dept. of Immunology, Faculty of Medicine, Shahed University, Persian Gulf highway, Postal Box: 159/18155, Postal code: 3319118651,
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hajatbeigi B, Hajighasemi F. Cytotoxicity of Metoprolol on Leukemic Cells in Vitro . IJBC. 2018; 10 (4) :124-129
URL: http://ijbc.ir/article-1-841-en.html


Volume 10, Issue 4 (December 2018 2018) Back to browse issues page
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3877