Volume 14, Issue 3 ( September 2022 2022)                   Iranian Journal of Blood and Cancer 2022, 14(3): 16-21 | Back to browse issues page

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Hashemi M, Esmaeili gouvarchin ghaleh H, Rostami S, Jalali kondori B. CD38 gene polymorphisms and genetic susceptibility to chronic lymphocytic leukemia. Iranian Journal of Blood and Cancer 2022; 14 (3) :16-21
URL: http://ijbc.ir/article-1-1275-en.html
CD38 gene polymorphisms and genetic susceptibility to chronic lymphocytic leukemia
Morteza Hashemi1 , Hadi Esmaeili gouvarchin ghaleh1 , Shahrbano Rostami2 , Bahman Jalali kondori * 3
1- Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran.
2- Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. , bahmanjalali2010@gmail.com
Abstract:   (871 Views)

Introduction: CLL is one of the most common leukemias, which is categorized by the accumulation of mature CD5+ B-lymphocytes in the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, the status of rs6449182 polymorphism of the CD38 gene and its association with clinical and laboratory parameters of CLL patients was evaluated.
 Methods: Genomic DNA extraction was performed using the salting out method. The CD38 gene polymorphism (rs6449182) was studied in 70 patients with CLL and 70 healthy individuals using the PCR-RFLP method.
Results: The results of this study showed that the control group had 86% wild-type rs6449182 (CC), 12% heterozygous (CG), and 2% homozygous (GG) genotypes. In the case group, 62% had wild-type genotype (CC) 26% were heterozygous (CG), and 12% were homozygous (GG). Statistical analysis showed that the heterozygous genotype for the CD38 gene  was significantly associated with CLL. It was also understood that this polymorphism had a significant relationship with hemoglobin, age, and organomegaly of patients.
Conclusions: The CD38 gene polymorphism of rs6449182 SNP G allele had the highest frequency. Moreover, based on the results, this polymorphism has a significant relationship with organomegaly, which indicates the importance of these markers in the pathogenesis and prognosis of the disease.
Keywords: Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38
 

Keywords: Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38
Full-Text [PDF 332 kb]   (489 Downloads)    
: Original Article | Subject: Immunology
Received: 2022/07/4 | Accepted: 2022/08/12 | Published: 2022/09/19
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