[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره كنفرانس :: ثبت نام :: ارسال مقاله :: برقراري ارتباط :: راهنماي پايگاه ::
:: دوره 10، شماره 2 - ( 3-1397 ) ::
جلد 10 شماره 2 صفحات 56-60 برگشت به فهرست نسخه ها
Frequency of BCR-ABL Fusion Transcripts in Iranian Azeri Turkish patients with Chronic Myeloid Leukemia
چکیده:   (251 مشاهده)
Background: The Philadelphia chromosome (Ph) characterized by t (9; 22) (q34; q11.2) is a reciprocal translocation giving rise to a chimeric BCR-ABL fusion gene. Incidence of Ph chromosome is over 98% in Patients with Chronic Myeloid Leukemia (CML) and around 20% in acute lymphoblastic leukemia (ALL). The finding of this fusion gene is essential for diagnosis of CML by detection of various fusion transcripts such as b2a2 and b3a2 transcripts and Ph positive ALL by detection of e1a2 (p190) transcripts. We conducted this study to determine the frequency of various BCR-ABL fusion transcripts in the west Azerbaijani patients with CML.
Methods: RNA was isolated from peripheral blood samples by standard protocols. BCR-ABL fusion gene detection was carried out with one-step multiplex RT-PCR in 41 west Azerbaijani patients with CML.
Results: Among patients with CML, the frequencies of b2a2 and b3a2 transcripts were 52.5% and 12.5%, respectively. Co-expression of b3a2 and b2a2 transcripts was found in 12.5% of the patients. 
Conclusion: The findings of this study showed that multiplex RT-PCR is a suitable technique to identify the typical BCR-ABL fusion transcripts in the west Azerbaijani patients with CML. Atypical transcripts possibly run away while using multiplex PCR.
متن کامل [DOC 180 kb]   (194 دریافت)    
: پژوهشي | موضوع مقاله: Adults Hematology & Oncology
دریافت: ۱۳۹۶/۱۲/۲۳ | پذیرش: ۱۳۹۷/۴/۲۵ | انتشار: ۱۳۹۷/۶/۴
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA code


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bagheri M, Abdi Rad I, Maleki D, Eishi A, Valizadeh N. Frequency of BCR-ABL Fusion Transcripts in Iranian Azeri Turkish patients with Chronic Myeloid Leukemia. IJBC. 2018; 10 (2) :56-60
URL: http://ijbc.ir/article-1-793-fa.html

Frequency of BCR-ABL Fusion Transcripts in Iranian Azeri Turkish patients with Chronic Myeloid Leukemia. مجله خون و سرطان ايران. 1397; 10 (2) :56-60

URL: http://ijbc.ir/article-1-793-fa.html



دوره 10، شماره 2 - ( 3-1397 ) برگشت به فهرست نسخه ها
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3764