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Volume 17, Issue 4 (December-2025 2025)
Iranian Journal of Blood and Cancer 2025, 17(4): 1-9 |
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Mehdizadeh F, Javidi-Aghdam K, Jafari-Sales A, Toofani Milani A, Pashazadeh M. Alteration of LncRNAs Expression Level in Blood of New Case and Medicated Behcet Patients as a Prognostic Biomarker. Iranian Journal of Blood and Cancer 2025; 17 (4) :1-9
URL: http://ijbc.ir/article-1-1808-en.html
URL: http://ijbc.ir/article-1-1808-en.html
Faezeh Mehdizadeh1 
, Kamran Javidi-Aghdam2 , Abolfazl Jafari-Sales3 , Attabak Toofani Milani4 , Mehrdad Pashazadeh *5
, Kamran Javidi-Aghdam2 , Abolfazl Jafari-Sales3 , Attabak Toofani Milani4 , Mehrdad Pashazadeh *5
1- Department of Microbiology, Ta.C., Islamic Azad University, Tabriz, Iran.
2- Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Microbiology, Ta.C., Islamic Azad University, Tabriz, Iran & Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran. & Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran.
4- Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran.
5- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran. & Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran. ,Mehrdadpashazadeh85@gmail.com
2- Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Microbiology, Ta.C., Islamic Azad University, Tabriz, Iran & Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran. & Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran.
4- Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran.
5- Infectious Diseases Research Center, TaMS.C., Islamic Azad University, Tabriz, Iran. & Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran. ,
Abstract: (321 Views)
Background: Behcet’s disease is a complex systemic inflammatory vasculitis marked by recurrent oral and genital ulcers, and uveitis. This study aims to evaluate the expression levels of long non-coding RNAs (LncRNAs) such as IFNG-AS1 and AC007278.2, along with the IL18 and IL18R1 genes, in the blood of active Behcet’s disease patients compared to HCs. Given the elevated levels of inflammatory cytokines in these patients, exploring the role of LncRNAs could provide insights into their involvement in the disease's pathogenesis and inflammatory response.
Methods: This case-control study involved 40 Behcet’s disease patients (20 medicated, 20 new cases) and 40 HCs. 2.5 ml venous blood of all subject were collected, and RNA extracted by using cDNA synthesis kit. Evaluation of genes expression level through qRT-PCR to analyze the expression levels of specific genes associated with inflammation, providing insights into Behçet's disease pathology
Results: Elevated expression of IL-18, IL-18R1, Lnc-AC007278.2, and Lnc-IFNG-AS1 showed in new case patients compare to medicated patients and HCs. ROC curve analysis demonstrated high diagnostic efficacy for these biomarkers, particularly IL18R1, indicating their potential for accurate patient identification.
Conclusion: This study highlights the potential of LncRNAs IFNG-AS1 and IL18 as non-invasive biomarkers for Behcet’s disease, offering insights into disease pathology and enhancing diagnostic accuracy, especially in new cases.
Methods: This case-control study involved 40 Behcet’s disease patients (20 medicated, 20 new cases) and 40 HCs. 2.5 ml venous blood of all subject were collected, and RNA extracted by using cDNA synthesis kit. Evaluation of genes expression level through qRT-PCR to analyze the expression levels of specific genes associated with inflammation, providing insights into Behçet's disease pathology
Results: Elevated expression of IL-18, IL-18R1, Lnc-AC007278.2, and Lnc-IFNG-AS1 showed in new case patients compare to medicated patients and HCs. ROC curve analysis demonstrated high diagnostic efficacy for these biomarkers, particularly IL18R1, indicating their potential for accurate patient identification.
Conclusion: This study highlights the potential of LncRNAs IFNG-AS1 and IL18 as non-invasive biomarkers for Behcet’s disease, offering insights into disease pathology and enhancing diagnostic accuracy, especially in new cases.
Keywords: Behcet’s disease, Autoimmune disorder, Long non-coding RNAs (LncRNAs), Real-Time PCR, Biomarkers, Polygenic disease
: Original Article |
Subject:
Pediatric Hematology & Oncology
Received: 2025/10/5 | Accepted: 2025/11/7 | Published: 2025/12/30
Received: 2025/10/5 | Accepted: 2025/11/7 | Published: 2025/12/30
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