[Home ] [Archive]    
:: Home :: About Journal :: Registration :: Article Submission :: Contact us :: Site Map ::
:: Volume 6, Issue 3 (Spring 2014) ::
IJBC 2014, 6(3): 103-107 Back to browse issues page
Comparative Study of Four Platelet-Rich Plasma Methods for Preparing Platelet Concentrates
Mojgan Pourmokhtar * , Ebadollah Ssalek Moghadam , Fatemeh Abbasi , Nasrin Zarei
Abstract:   (4441 Views)
Background: Platelet preparations have been used for a wide variety of clinical applications such as hemorrhage, tissue engineering and cellular therapy. Platelet concentrates can be prepared by the apheresis method or from the whole blood using the Buffy-coat or Platelet-rich plasma methods. The purpose of this study was to compare four variations of platelet-rich plasma method based on double centrifugation protocol to identify the optimal centrifugation conditions with greatest platelet recovery and highest enrichment capacity for preparing platelet concentrates. Materials and Methods: Blood samples were obtained from 145 donors, chosen randomly from the donation department at the Tehran Blood Transfusion Center, Tehran, Iran. Four variations of platelet-rich plasma methods were selected for preparation of platelet concentrates. Platelet counting analysis was performed on samples and platelet enrichment and platelet recovery were calculated by investigating the correlation between the number of platelets in the whole blood, platelet-rich plasma and platelet concentrates. Results: Platelet count analysis revealed that the method performed with 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step had the highest platelet enrichment (5.59fold) and greatest platelet recovery (78.63%). Conclusion: Within the limits of this study, it can be concluded that 2100 ×g for 2.30 min in the first centrifugation step and 4150 ×g for 6 min in the second centrifugation step yielded the greatest platelet recovery and highest enrichment capacity and is a good choice for preparing platelet concentrates. Keywords: Platelet, concentrates, plasma, centrifugation, recovery, enrichment.
Keywords: Platelet, concentrates, plasma, centrifugation, recovery, enrichment
Full-Text [PDF 592 kb]   (3025 Downloads)    
: Research | Subject: Pediatric Hematology & Oncology
Received: 2013/08/13 | Accepted: 2013/12/3 | Published: 2014/04/8
Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pourmokhtar M, Ssalek Moghadam E, Abbasi F, Zarei N. Comparative Study of Four Platelet-Rich Plasma Methods for Preparing Platelet Concentrates. IJBC. 2014; 6 (3) :103-107
URL: http://ijbc.ir/article-1-456-en.html


Volume 6, Issue 3 (Spring 2014) Back to browse issues page
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3764