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:: Volume 11, Issue 3 (September 2019 2019) ::
IJBC 2019, 11(3): 101-108 Back to browse issues page
Immunophenotyping of Nodal Peripheral T-cell Lymphomas and its Association with Epstein-Barr Virus
Bidish Kumar Patel, Debdatta Basu, Rakhee Kar, Biswajit Dubashi *
Additional Professor of Medical Oncology
Abstract:   (763 Views)
Background: Immunophenotyping in the rare group of nodal Peripheral T-cell Lymphomas (PTCL) exposes interesting features such as T-cell marker downregulation and paradoxically, the presence of reactive, clustered large-sized CD20 positive B-cells (B-cell proliferation). Epstein-Barr virus (EBV) has been suggested as a putative etiology in pathogenesis of B-cell lymphoma. We aimed to review the immunohistochemical profile of patients with nodal PTCL with emphasis on T-cell markers and immunophenotypic aberrations, CD20 positive large B-cells, Ki-67 scores (as a measure of proliferation index) and to assess the association of Epstein-Barr virus in various subtypes of nodal PTCL.
Methods: 80 cases of nodal PTCL diagnosed during January 2008-June 2013 were included in the study. Relevant clinical and hematological data were collected. Using Streptavidin-Biotin-Peroxidase system, staining for CD2, CD3, CD4, CD5, CD7, CD8, CD20,EBV-LMP1 and Ki-67 were performed on all blocks.CD10,CD23,Bcl-6, CD30 and ALK-1 were used in relevant cases.
Results: 95% of patients had downregulation of at least one T-cell marker (maximum: CD7 (87%), minimum: CD3 and CD5- 9% each). 29 patients (36%) showed markers of B-cell proliferation. Only five patients (6%) were positive for EBV-LMP1. There was a significant association between EBV-LMP1 positivity and B-cell proliferation (P=0.002). 17 patients (21%) had high Ki-67 index (≥80%).
Conclusion: Nodal PTCL showed frequent downregulation of T-cell markers. EBV was only infrequently positive in these Lymphomas. Clusters of large B-cells need to be noted in pathology reports and EBV needs to be tested for in such cases.
Keywords: Peripheral T-cell lymphoma, Immunophenotyping, Epstein-Barr virus, Angioimmunoblastic T-cell lymphoma, Anaplastic large cell lymphoma, Anaplastic lymphoma kinase
Full-Text [PDF 2544 kb]   (192 Downloads)    
: Research | Subject: Pathology
Received: 2019/03/18 | Accepted: 2019/09/13 | Published: 2019/10/8
* Corresponding Author Address: Department of Medical Oncology, JIPMER, Puducherry, INDIA. Pin - 605006
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Patel B K, Basu D, Kar R, Dubashi B. Immunophenotyping of Nodal Peripheral T-cell Lymphomas and its Association with Epstein-Barr Virus. IJBC. 2019; 11 (3) :101-108
URL: http://ijbc.ir/article-1-884-en.html

Volume 11, Issue 3 (September 2019 2019) Back to browse issues page
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
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