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:: Volume 13, Issue 3 (September 2021 2021) ::
IJBC 2021, 13(3): 85-91 Back to browse issues page
Evaluation of Lipid Peroxidation, Antioxidant Status and Trace Elements in Red Blood Cell Concentrates during Storage
Sara Danehpash, Hamid Shirkhanloo, Kian Azami, Mohammad Reza Deyhim *
Blood Transfusion Research Center, High Institute for Research and Education for Transfusion Medicine, Tehran, Iran
Abstract:   (1270 Views)
Background: Red blood cell concentrates (RBCs) undergo biochemical and structural changes during storage, commonly referred to as the RBC storage lesion (RSL) which reduces the survival of RBCs and affect transfusion efficiency. Lipid peroxidation and oxidative damage are the most important side effect of RSL. We aimed to evaluate oxidative damage and some related parameters in RBCs during storage. 
Methods: In this experimental study, eight RBCs bags were randomly selected from healthy blood donors and stored at 2-6 °C for 35 days. Oxidative stress markers, trace elements and RBCs metabolism parameters including total antioxidant capacity (TAC), malondialdehyde (MDA), zinc, copper, manganese, selenium, iron, magnesium, sodium, potassium, lactate, glucose, lactate dehydrogenase (LDH) enzyme activity, pH and also RBCs hematological indexes including hemoglobin, hematocrit, MCV, MCH, MCHC and free plasma hemoglobin were evaluated during of RBCs storage. 
Results: The results showed a significant increase in hemoglobin, hematocrit, calcium, phosphorus, iron, magnesium, lactate, potassium, free plasma hemoglobin, TAC and LDH activity during RBCs storage according to one way analysis of variance (P<0.05), while a significant decrease was shown in pH, sodium and glucose concentration (P<0.05). No significant mean changes were seen in MDA, zinc, copper, manganese and selenium concentration during RBC storage.
Conclusion: It seems that RBCs at the end of the storage period have a lower quality than newly prepared ones. Therefore, we commend that RBC products rather be used before third week of storage due to post transfusion side effects in blood recipients. 
Keywords: Red blood cell concentrate, Red blood cell storage lesion, Oxidative damage, Lipid peroxidation, Antioxidant status, Trace elements
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: Research | Subject: Methodology
Received: 2020/11/26 | Accepted: 2021/07/4 | Published: 2022/02/13
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Danehpash S, Shirkhanloo H, Azami K, Deyhim M R. Evaluation of Lipid Peroxidation, Antioxidant Status and Trace Elements in Red Blood Cell Concentrates during Storage. IJBC. 2021; 13 (3) :85-91
URL: http://ijbc.ir/article-1-1089-en.html

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Volume 13, Issue 3 (September 2021 2021) Back to browse issues page
مجله ی خون و سرطان ایران Iranian Journal of Blood and Cancer
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