P Shayan, M Izadyar, B Eckert,
Volume 4, Issue 3 (Spring 2012)
Abstract
Objective: To study whether the generated CD1a positive cells belong to the leukemic cells among patients with juvenile myelomonocytic leukemia.
Materials and Methods: We used mononuclear cells from 3 patients with juvenile myelomonocytic leukemia, from which two had monosomy 7. The mononuclear cells from these patients were cultured in RPMI/10%FCS without adding exogeneous growth factors for 7 days. At day 7 the cultured cells were harvested and analyzed using antibodies against Ki 67, CD20 and CD1a. Additionally the cultured cells were analyzed using antibodies against CD1a and CD20 and chromosome 7 specific DNA probe, using combined fluorescence immunophenotyping and interphase cytogenetic techniques.
Results: The immunocytochemistry assay demonstrated that a high number of cells were in proliferation status, which was determined by antibody against proliferation associated nuclear protein ki 67. The percentage of Ki 67 positive cells was between 24% and 38% respectively. The percentage of CD1a positive cells was between 8% and 31% and the percentage of CD20 positive cells was between 5% and 12% respectively. The fluorescence immunophenotyping and interphase cytogenetic analysis showed that nearly all CD1a positive cells of one patient with monosomy 7 had one chromosome 7, whereas in other patient with monosomy 7, the amount of CD1a positive cells having only one chromosome 7 was approximately 11%. Furthermore, the combined immunophenotyping and cytogenetic analysis showed that the CD20 positive cells in all patients had normal karyotype.
Conclusion: Our results suggest that CD1a positive cells generated by mononuclear cells from patients with juvenile myelomonocytic leukemia in vitro most probably belong to the leukemic cells. Since monosomy 7 could not be detected in all CD1a positive cells in juvenile myelomonocytic leukemia patients with monosomy 7, it is to assume that monosomy 7 is a secondary event in the pathophysiology of juvenile myelomonocytic leukemia.
Keywords: Leukemia, mononuclear cells, CD1a antigen, chromosome 7, monosomy, cell proliferation, myelomonocytic, juvenile
Amir Ataolah Hiradfar, Azim Rezamand, Matin Pourghasem, Sepideh Karkon Shayan, Behnaz Ghamari, Farid Karkon Shayan,
Volume 10, Issue 1 ( March 2018 2018)
Abstract
Background: In patients undergoing chemotherapy for cancer, anemia is part of the progression of the disease. Considering the effects of anemia on quality of life of the patients, the prevention and treatment of chemotherapy-induced anemia is crucial. This study was aimed to evaluate the efficacy of recombinant human erythropoietin in reducing the need for blood transfusion in children with solid tumors receiving chemotherapy.
Methods: In a clinical trial, 57 children referred to the pediatric centre of Tabriz University of Medical Sciences with a diagnosis of solid tumor were randomly assigned into two groups. The intervention group (n=29) received recombinant human erythropoietin (rHuEPO) at a dose of 450 IU/kg subcutaneously once a week for 12 weeks, and the control group (n=28) received no intervention in this regard. Hemoglobin levels were analysed at the beginning and end of the study. The need for blood transfusion was also assessed in the patients.
Results: The mean Hb at the beginning of the study was 8.85±1.01 and 8.98±0.11 g in the intervention and control groups, respectively. The mean Hb at the end of the study was 9.78±0.32 g/dl in the intervention group and 7.79±0.24 g/dl in the control group. Hb level was significantly higher in the intervention group at the end of the 12 weeks of treatment with rHuEPO than in the control group (P=0.001).
Conclusion: Based on the results of this study, rHuEPO administration seems to be beneficial in the prevention and treatment of chemotherapy-induced anemia in children with solid tumors and reduces the amount of blood transfusion in these patients.
