Volume 14, Issue 3 ( September 2022 2022)                   Iranian Journal of Blood and Cancer 2022, 14(3): 16-21 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Hashemi M, Esmaeili gouvarchin ghaleh H, Rostami S, Jalali kondori B. CD38 gene polymorphisms and genetic susceptibility to chronic lymphocytic leukemia. Iranian Journal of Blood and Cancer 2022; 14 (3) :16-21
URL: http://ijbc.ir/article-1-1275-en.html
CD38 gene polymorphisms and genetic susceptibility to chronic lymphocytic leukemia
Morteza Hashemi1 , Hadi Esmaeili gouvarchin ghaleh1 , Shahrbano Rostami2 , Bahman Jalali kondori * 3
1- Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, Iran.
2- Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. , bahmanjalali2010@gmail.com
Abstract:   (1142 Views)

Introduction: CLL is one of the most common leukemias, which is categorized by the accumulation of mature CD5+ B-lymphocytes in the peripheral blood, bone marrow, and secondary lymphoid organs. In this study, the status of rs6449182 polymorphism of the CD38 gene and its association with clinical and laboratory parameters of CLL patients was evaluated.
 Methods: Genomic DNA extraction was performed using the salting out method. The CD38 gene polymorphism (rs6449182) was studied in 70 patients with CLL and 70 healthy individuals using the PCR-RFLP method.
Results: The results of this study showed that the control group had 86% wild-type rs6449182 (CC), 12% heterozygous (CG), and 2% homozygous (GG) genotypes. In the case group, 62% had wild-type genotype (CC) 26% were heterozygous (CG), and 12% were homozygous (GG). Statistical analysis showed that the heterozygous genotype for the CD38 gene  was significantly associated with CLL. It was also understood that this polymorphism had a significant relationship with hemoglobin, age, and organomegaly of patients.
Conclusions: The CD38 gene polymorphism of rs6449182 SNP G allele had the highest frequency. Moreover, based on the results, this polymorphism has a significant relationship with organomegaly, which indicates the importance of these markers in the pathogenesis and prognosis of the disease.
Keywords: Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38
 

Keywords: Polymorphism, Single Nucleotide Polymorphisms, Chronic lymphocytic leukemia (CLL), CD38
Full-Text [PDF 332 kb]   (743 Downloads)    
: Original Article | Subject: Immunology
Received: 2022/07/4 | Accepted: 2022/08/12 | Published: 2022/09/19
References
1. Al-Kahiry, WM., et al., Prognostic Impact of Interleukin-10 in Patients with Chronic Lymphocytic Leukemia. Iran J Blood Cancer. 2021; 13 (1) :11-16.
2. Falay, M., et al., Immunophenotyping of chronic lymphocytic leukemia. Clin Lab. 2017;63(10):1621-6. [DOI:10.7754/Clin.Lab.2017.170406]
3. Alqasim, AMZ., et al., Flowcytometric Measurement of CD5, CD23, and CD38 expression as a diagnostic and prognostic markers in CLL patients. Iraqi j hematol. 2015;4(1):16-38.
4. Nørgaard, CH., et al., Subtype assignment of CLL based on B-cell subset associated gene signatures from normal bone marrow-A proof of concept study. PloS one. 2018;13(3):e0193249. [DOI:10.1371/journal.pone.0193249]
5. Bürgler, S., et al., CD38 in chronic lymphocytic leukemia (CLL)-from a diagnostic tool to a therapeutic target. J Blood Disord Med. 2016;1(2). [DOI:10.16966/2471-5026.e103]
6. Higashida, H., et al., Social memory, amnesia, and autism: brain oxytocin secretion is regulated by NAD+ metabolites and single nucleotide polymorphisms of CD38. Neurochem Int. 2012;61(6):828-38. [DOI:10.1016/j.neuint.2012.01.030]
7. Hashemi, M., et al., Association between rs 1800561 CD38 Gene Polymorphism and Chronic Lymphocytic Leukemia Risk. J Mazandaran Univ Med Sci. 2020; 30 (191) :102-108.
8. Huq, MA., et al., Identification of functional SNPs in genes and their effects on plant phenotypes. J Plant Biotechnol. 2016;43(1):1-11. [DOI:10.5010/JPB.2016.43.1.1]
9. Mokrane, А., et al., Genetic Variants of SNPs RS6449182 CD38 Gene: Correlation with Age, Sex, and Tumor Stage in Patients with Colorectal Cancer. Opera Medica et Physiologica. 2016(3-4).
10. Rai, KR., et al., Advances in the clinical staging of chronic lymphocytic leukemia. Clin Chem. 2011;57(12):1771-2. [DOI:10.1373/clinchem.2010.159004]
11. Suguna, S., et al., Genomic DNA isolation from human whole blood samples by non enzymatic salting out method. Int J pharm pharm sci. 2014;6(6):198-9.
12. Djadid, ND., et al., A simple and rapid nested polymerase chain reaction-restriction fragment length polymorphism technique for differentiation of pathogenic and nonpathogenic Leptospira spp. Diagn Microbiol Infect Dis. 2009;63(3):251-6. [DOI:10.1016/j.diagmicrobio.2008.10.017]
13. Crassini, K., et al., Molecular pathogenesis of chronic lymphocytic leukaemia. Br J Haematol. 2019;186(5):668-84. [DOI:10.1111/bjh.16102]
14. Fischer, K., et al., Optimizing frontline therapy of CLL based on clinical and biological factors. Hematology 2014, the American Society of Hematology Education Program Book. 2017;2017(1):338-45. [DOI:10.1182/asheducation-2017.1.338]
15. Lee, MR., et al., A role for the CD38 rs3796863 polymorphism in alcohol and monetary reward: evidence from CD38 knockout mice and alcohol self-administration,[11C]-raclopride binding, and functional MRI in humans. Am J Drug Alcohol Abuse. 2020;46(2):167-79. [DOI:10.1080/00952990.2019.1638928]
16. Malavasi, F., et al., Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev. 2008;88(3):841-86. [DOI:10.1152/physrev.00035.2007]
17. Hallek, M., Chronic lymphocytic leukemia: 2017 update on diagnosis, risk stratification, and treatment. Am J Hematol. 2017;92(9):946-65. [DOI:10.1002/ajh.24826]
18. Delgado García, A., The relationship between the polymorphism (rs6449182) of CD38 and the prognosis of patients with chronic lymphocytic leukemia treated with fludarabine, cyclophosphamide and rituximab (FCR): a 4 year prospective cohort study. 2016.
19. Chong, A., et al., ADP ribosyl-cyclases (CD38/CD157), social skills and friendship. Psychoneuroendocrinology. 2017;78:185-92. [DOI:10.1016/j.psyneuen.2017.01.011]
20. Jamroziak, K., et al., CD38 gene polymorphisms contribute to genetic susceptibility to B-cell chronic lymphocytic leukemia: evidence from two case-control studies in Polish Caucasians. Cancer Epidemiol Biomarkers Prev. 2009;18(3):945-53. [DOI:10.1158/1055-9965.EPI-08-0683]
21. Deaglio, S., et al., CD38 is a signaling molecule in B-cell chronic lymphocytic leukemia cells. Blood. 2003;102(6):2146-55. [DOI:10.1182/blood-2003-03-0989]
22. Aydin, S., et al., CD38 gene polymorphism and chronic lymphocytic leukemia: a role in transformation to Richter syndrome? Blood. 2008;111(12):5646-53. [DOI:10.1182/blood-2008-01-129726]
23. Eyada, TK., et al. CD38 Gene polymorphisms and susceptibility to B cell chronic lymphocytic leukemia. Comp Clin Path. 2013;22(4):573-9. [DOI:10.1007/s00580-012-1447-0]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 All Rights Reserved | Iranian Journal of Blood and Cancer

Designed & Developed by : Yektaweb