Volume 15, Issue 4 (September 2023 2023)                   Iranian Journal of Blood and Cancer 2023, 15(4): 253-261 | Back to browse issues page

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Mansouri V, Zamanian Azodi M, M Robati R, Razzagh Z, Arjmand B, Rezaei Tavirani M et al . Digging deep into molecular pathways: Investigating the effects of 9S-HOD on leukemia cells using a systems biology approach. Iranian Journal of Blood and Cancer 2023; 15 (4) :253-261
URL: http://ijbc.ir/article-1-1399-en.html
Digging deep into molecular pathways: Investigating the effects of 9S-HOD on leukemia cells using a systems biology approach
Vahid Mansouri1 , Mona Zamanian Azodi * 2, Reza M Robati3 , Zahra Razzagh4 , Babak Arjmand5 , Mostafa Rezaei Tavirani6 , Mohammad Rostami Nejad7
1- Proteomics research center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Proteomics research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , mona.azodi@gmail.com
3- Skin research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5- Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. and Iranian Cancer Control Center (MACSA), Tehran, Iran
6- Proteomics research center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
7- Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:   (641 Views)

Background: Acute myeloid leukemia (AML) is a malignant disorder characterized by a poor prognosis. Current therapeutic approaches include chemotherapy, steroids administration, and blood transfusion. Previous studies have highlighted the potential anticancer property of 9-hydroxyoctadecadienoic acid (9S-HOD). This molecular computational research aims to investigate the intricate molecular mechanism underlying the effects of 9S-HOD on leukemia cells.
Methods: Utilizing proteomic data and the optimum numbers of the first neighbors from the STRING database, Cytoscape 3.9.1 along with its applications, NetworkAnalyzer and ClueGO+CuePedia were employed to analyze the constructed protein-protein interaction (PPI) network, its centrality and enrichments.
Results: The analysis identified five proteins namely ACTB, HSP90AA1, GAPDH, TP53, and HSP90AB1 as potential central nodes within the PPI network. Furthermore, gene ontology analysis revealed “Response to salt stress” and “Positive regulation of type 1 interferon production” as enriched biological processes associated with these key elements of the PPI network. HSPA8, MYC, and KAT5 were identified as seed proteins within the sub-networks.
Conclusion: The findings suggest that the effect of 9S-HOD on the leukemia cells primarily involves the regulation of ACTB, HSP90AA1, HSP90AB1, GAPDH, and TP53.  additionally, HSPA8, MYC, and KAT5 emerged as important proteins influenced by 9S-HOD.

Keywords: 9-hydroxyoctadecadienoic acid (9S-HOD), Leukemia cells, Protein-protein interaction network analysis, Centrality, Gene ontology.
Full-Text [PDF 2968 kb]   (281 Downloads)    
: Original Article | Subject: Genetics
Received: 2023/06/30 | Accepted: 2023/09/2 | Published: 2023/09/17
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